Translational_Unit

Part:BBa_K1461310

Designed by: Shunsuke Sumi   Group: iGEM14_UT-Tokyo   (2014-09-26)

pCMV-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term

This is a GFP generator. This generator is used for characterization of translation inhibition by miR-142-3p and miR-142-5p in HL60. This contains pCMV, RBS, GFP, 2 miR-142-3p binding sites, 2 miR-142-5p binding sites and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1250


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Categories
Parameters
None